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Image Search Results
Journal: PLoS ONE
Article Title: Transgenic Expression of Entire Hepatitis B Virus in Mice Induces Hepatocarcinogenesis Independent of Chronic Liver Injury
doi: 10.1371/journal.pone.0026240
Figure Lengend Snippet: The HBV integration sites are identified for Mutant 1-Line 7 ( A–C ), Mutant 1-Line 4 ( D–F ), and wildtype Tg05 ( G - I ) mice. ( A , D , and G ) Detection of an end of the HBV transgene and its flanking mouse sequence by PCR. Lane 1, 1 kb Plus DNA size ladder (Fermentas); lane 2, PCR product. ( B , E , and H ) Analysis of the integration sites by fluorescent in situ hybridization (FISH). The metaphase cell was stained with an HBV probe (red arrow) and with DAPI (blue). The cell in ( B ) was also stained with a probe (green arrow) derived from the mouse BAC clone RP23-355K3 that corresponds to band E1 of chromosome 11 (11E1). ( C , F , and I ) Cytogenetic localization of the HBV transgenes. Shown from left to right are the mouse chromosome ideograms, the corresponding G-banded chromosomes, and the DAPI-banded chromosomes with HBV signal from the FISH figures. RP23-355K3 was hybridized to 11qE1 and cross-hybridized to chromosome 6 at band B1. The integrated HBV gene (red arrow) was localized to chromosome 11E1 in Mutant 1-Line 7, to chromosome 1F in Mutant 1-Line 4, and to chromosome 11B5 in wildtype Tg05 mice.
Article Snippet: These FISH probes were mixed with sheared
Techniques: Mutagenesis, Sequencing, In Situ Hybridization, Staining, Derivative Assay